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Determination of N-acetyl-S-(N-alkylthiocarbamoyl)-L-cysteine a principal metabolite of alkyl isothiocyanates in rat urine
Summary
Type:
HPLC
Application #:
103590
Catalog#:
720022.40 - MN HPLC Column, Nucleosil C8, 100A, 10um, 4x250mm
Detection:
UV, 240nm
Conditions:
eluent: methanol - water (1:1, v/v)
flow rate 1.5ml/min
Authors:
Mennicke WH
Source:
J Chromatogr 414 (1987) 19 - 24
Details
Legend: Fig. 2. Chromatograms of an extract of A) blank rat urine, B) blank rat urine after addition of 11.1µg of mercapturic acid (as the dicyclohexylamine salt, equivalent to 5.0µg of N-benzyl-N'-n-butylt
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